Yes, but as far as I'm aware it would require a little bit of...

Typically, enemies will not respawn after they are killed by Mario, either via stomping, fireball, or being thrown in a substance like lava or poison water.
Enemy spawning however, is not affected when the enemy is knocked off of an edge.

So, what's the solution? Well, I would try and create a zone-controlled sprite for what enemies you are trying to affect. Create a zone for each one of these enemies, or simply just create one zone for a group of enemies you'd want to despawn after leaving the zone. Then you would need a custom actor to control and enable the zone to function this way. This would unfortunately either have to be programmed by yourself, or you can request it on this site or on the NSMB Central discord.

Sorry it isn't easier
Luckily, I don't think it would be too hard to program.

Someone can correct me if I'm wrong about any of this however...